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<oaf:result xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
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xmlns:oaf="http://namespace.openaire.eu/oaf"
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xsi:schemaLocation="http://namespace.openaire.eu/oaf http://datathon.openaire.eu/datathon-schema/oaf-dataset-1.1.xsd">
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<oaf:language code="und">Undetermined</oaf:language>
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<oaf:dateofacceptance>2017-01-01</oaf:dateofacceptance>
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<oaf:size/>
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<oaf:resulttype>dataset</oaf:resulttype>
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<oaf:publisher>Figshare</oaf:publisher>
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<oaf:resulttitle>Transcriptomic responses of water buffalo liver
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to infection with the digenetic fluke Fasciola gigantica</oaf:resulttitle>
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<oaf:resourcetype>Collection</oaf:resourcetype>
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<oaf:version/>
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<oaf:subject type="mesheuropmc" provenance="inference">food and beverages</oaf:subject>
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<oaf:subject type="keyword">Microbiology</oaf:subject>
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<oaf:subject type="keyword">Computational
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Biology</oaf:subject>
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<oaf:subject type="keyword">Virology</oaf:subject>
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<oaf:subject type="keyword">Immunology</oaf:subject>
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<oaf:subject type="keyword">29999 Physical Sciences not
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elsewhere classified</oaf:subject>
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<oaf:subject type="keyword">Space Science</oaf:subject>
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<oaf:subject type="keyword">Genetics</oaf:subject>
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<oaf:subject type="keyword">69999 Biological Sciences
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not elsewhere classified</oaf:subject>
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<oaf:subject type="keyword">Infectious
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Diseases</oaf:subject>
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<oaf:subject type="mesheuropmc" provenance="inference">parasitic diseases</oaf:subject>
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<oaf:subject type="mesheuropmc" provenance="inference">animal diseases</oaf:subject>
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<oaf:subject type="keyword">Cancer</oaf:subject>
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<oaf:format/>
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<oaf:description>Abstract Background Fasciola gigantica, the tropical liver fluke,
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infects buffaloes in Asian and African countries and causes significant economic
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losses and poses public health threat in these countries. However, little is
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known of the transcriptional response of buffaloes to infection with F.
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gigantica. The objective of the present study was to perform the first
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transcriptomic analysis of buffalo liver infected by F. gigantica. Understanding
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the mechanisms that underpin F. gigantica infection in buffaloes will contribute
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to our ability to control this parasite. Methods We challenged buffaloes with
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500 viable F. gigantica metacercariae and collected liver samples through a time
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course at 3, 42 and 70Â days post-infection (dpi). Then, we performed gene
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expression analysis on liver samples using RNA sequencing (RNA-Seq) Illumina
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technology and confirmed the RNA-Seq data by quantitative RT-PCR analysis.
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Results Totals of 496, 880 and 441 differentially expressed transcripts were
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identified in the infected livers at 3, 42 and 70 dpi, respectively. Gene
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Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis
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revealed that transcriptional changes in the liver of infected buffaloes evolve
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over the course of infection. The predominant response of buffaloes to infection
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was mediated by certain pathways, such as MHC antigen processing and
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presentation, Toll-like receptor 4 (TLR4), transforming growth factor beta
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(TGF-β), and the cytochrome P450. Hepatic drug metabolizing enzymes and bile
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secretion were also affected. Conclusions Fasciola gigantica can induce
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statistically significant and biologically plausible differences in the hepatic
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gene expression of infected buffaloes. These findings provide new insights into
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the response of buffaloes to F. gigantica over the course of infection, which
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may be useful in determining pathways that can modulate host-parasite
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interaction and thus potentially important for clearance of the
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parasite.</oaf:description>
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<oaf:embargoenddate/>
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<oaf:device/>
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<oaf:collectedfrom name="Datacite"/>
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<oaf:pid type="doi">10.6084/m9.figshare.c.3678754</oaf:pid>
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<oaf:originalId>10.6084/m9.figshare.c.3678754</oaf:originalId>
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<oaf:originalId>http://dx.doi.org/10.6084/m9.figshare.c.3678754</oaf:originalId>
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<oaf:creator rank="4">Jun-Jun He</oaf:creator>
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<oaf:creator rank="9">Ai-Jiang Guo</oaf:creator>
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<oaf:creator rank="7">Jian-Gang Ma</oaf:creator>
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<oaf:creator rank="2">Xiao-Xuan Zhang</oaf:creator>
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<oaf:creator rank="10">Xing-Quan Zhu</oaf:creator>
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<oaf:creator rank="8">Wei-Yi Huang</oaf:creator>
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<oaf:creator rank="6">Wen-Bin Zheng</oaf:creator>
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<oaf:creator rank="5">Zhao-An Sheng</oaf:creator>
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<oaf:creator rank="3">Hany Elsheikha</oaf:creator>
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<oaf:creator rank="1">Fu-Kai Zhang</oaf:creator>
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<oaf:relationships>
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<oaf:relationship semantics="isRelatedTo" provenance="inference">
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<oaf:target type="result" id="dedup_wf_001::b9d8f5a45247260d099c5900a9a9b6ab">
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<oaf:publisher>BioMed Central</oaf:publisher>
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<oaf:dateofacceptance>2017-02-01</oaf:dateofacceptance>
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<oaf:resulttitle>Transcriptomic responses of water buffalo liver to infection with the
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digenetic fluke Fasciola gigantica</oaf:resulttitle>
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<oaf:resulttype>publication</oaf:resulttype>
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</oaf:target>
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</oaf:relationship>
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</oaf:relationships>
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<oaf:instances>
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<oaf:instance>
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<oaf:hostedby name="figshare"/>
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<oaf:instancetype>Collection</oaf:instancetype>
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<oaf:accessrights code="UNKNOWN">UNKNOWN</oaf:accessrights>
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<oaf:webresource>
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<oaf:url>http://dx.doi.org/10.6084/m9.figshare.c.3678754</oaf:url>
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</oaf:webresource>
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</oaf:instance>
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</oaf:instances>
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</oaf:result>
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