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xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
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<header>
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<objIdentifier>dedup_wf_001::0472bc7a1f3c9afeab815dfc50137cdc
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</objIdentifier>
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<dateOfCollection>2015-02-06T16:52:05Z</dateOfCollection>
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<dateOfTransformation>2016-02-06T16:52:05Z</dateOfTransformation>
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<counters>
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<counter_authorship value="6" />
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<counter_dedup value="2" />
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<metadata>
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<oaf:entity xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
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xmlns:oaf="http://namespace.openaire.eu/oaf"
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xsi:schemaLocation="http://namespace.openaire.eu/oaf http://www.openaire.eu/schema/0.2/oaf-0.2.xsd">
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<result>
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<subject schemename="dnet:result_subject" classname="keyword"
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schemeid="dnet:result_subject" classid="keyword">DOAJ:Health Sciences
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</subject>
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<subject schemename="dnet:result_subject" classname="keyword"
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schemeid="dnet:result_subject" classid="keyword">Pathology</subject>
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<subject schemename="dnet:result_subject" classname="keyword"
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schemeid="dnet:result_subject" classid="keyword">DOAJ:Pathology</subject>
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<subject schemename="dnet:result_subject" classname="keyword"
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schemeid="dnet:result_subject" classid="keyword">DOAJ:Medicine (General)
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</subject>
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<subject schemename="dnet:result_subject" classname="keyword"
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schemeid="dnet:result_subject" classid="keyword">R</subject>
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<subject schemename="dnet:result_subject" classname="keyword"
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schemeid="dnet:result_subject" classid="keyword">Medicine</subject>
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<subject schemename="dnet:result_subject" classname="keyword"
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schemeid="dnet:result_subject" classid="keyword">Research Article</subject>
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<subject schemename="dnet:result_subject" classname="keyword"
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schemeid="dnet:result_subject" classid="keyword">RB1-214</subject>
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37
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<title schemename="dnet:dataCite_title" classname="main title"
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schemeid="dnet:dataCite_title" classid="main title">Effects of EDTA and Sodium
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39
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Citrate on hormone measurements by fluorometric (FIA) and
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immunofluorometric (IFMA) methods
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</title>
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42
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<dateofacceptance>2002-05-23</dateofacceptance>
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43
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<publisher>BioMed Central</publisher>
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<resulttype schemename="dnet:result_typologies"
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classname="publication" schemeid="dnet:result_typologies" classid="publication" />
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<language schemename="dnet:languages" classname="English"
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schemeid="dnet:languages" classid="eng" />
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<journal eissn="1472-6890" issn="1472-6890" lissn="">BMC
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Clinical Pathology
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</journal>
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<description>
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<p>Abstract</p>
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<p>Background</p>
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<p>Measurements of hormonal concentrations by immunoassays using
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fluorescent tracer substance (Eu3+) are susceptible to the action
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of chemical agents that may cause alterations in its original
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structure. Our goal was to verify the effect of two types of
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anticoagulants in the hormone assays performed by fluorometric
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(FIA) or immunofluorometric (IFMA) methods.
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</p>
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<p>Methods</p>
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<p>
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Blood samples were obtained from 30 outpatients and were drawn in
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EDTA, sodium citrate, and serum separation Vacutainer
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<sup>®</sup>
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Blood Collection Tubes. Samples were analyzed in automatized
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equipment AutoDelfia™ (Perkin Elmer Brazil, Wallac, Finland) for
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the following hormones: Luteinizing hormone (LH), Follicle
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stimulating homone (FSH), prolactin (PRL), growth hormone (GH),
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Sex hormone binding globulin (SHBG), thyroid stimulating hormone
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(TSH), insulin, C peptide, total T3, total T4, free T4,
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estradiol,
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progesterone, testosterone, and cortisol. Statistical analysis was
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carried out by Kruskal-Wallis method and Dunn's test.
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</p>
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<p>Results</p>
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<p>No significant differences were seen between samples for LH,
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FSH, PRL and free T4. Results from GH, TSH, insulin, C peptide,
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79
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SHBG, total T3, total T4, estradiol, testosterone, cortisol, and
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80
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progesterone were significant different between serum and
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EDTA-treated samples groups. Differences were also identified
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between serum and sodium citrate-treated samples in the analysis
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for TSH, insulin, total T3, estradiol, testosterone and
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progesterone.
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85
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</p>
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<p>Conclusions</p>
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<p>We conclude that the hormonal analysis carried through by FIA
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or
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IFMA are susceptible to the effects of anticoagulants in the
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90
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biological material collected that vary depending on the type of
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assay.
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</p>
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</description>
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<source>BMC Clinical Pathology, Vol 2, Iss 1, p 2 (2002)</source>
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schemeid="dnet:access_modes" classid="OPEN" />
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<datainfo>
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<trust>0.9</trust>
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<fullname>Leme Cassia</fullname>
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<ranking>5</ranking>
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<fullname>Kohek Maria</fullname>
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<ranking>1</ranking>
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<fullname>Lando Valeria</fullname>
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<ranking>3</ranking>
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<fullname>Nakamura Izabel T</fullname>
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<ranking>6</ranking>
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<fullname>Mendonca Berenice B</fullname>
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<ranking>2</ranking>
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<fullname>de Oliveira Suzimara A</fullname>
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<children>
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schemeid="dnet:dataCite_title" classid="main title">Effects of EDTA and
|
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185
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Sodium
|
|
186
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Citrate on hormone measurements by fluorometric (FIA) and
|
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187
|
immunofluorometric (IFMA) methods
|
|
188
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</title>
|
|
189
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<dateofacceptance>2002-05-01</dateofacceptance>
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190
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<publisher>BioMed Central</publisher>
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schemeid="dnet:dataCite_title" classid="main title">Effects of EDTA and
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198
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Sodium
|
|
199
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Citrate on hormone measurements by fluorometric (FIA) and
|
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200
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immunofluorometric (IFMA) methods
|
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201
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</title>
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202
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<dateofacceptance>2002-05-23</dateofacceptance>
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